Product Name
CD40 Ligand, Monoclonal Antibody
Popular Item
Full Product Name
Hamster Anti-Mouse CD40 Ligand
Product Synonym Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
Product Gene Name
anti-CD40 Ligand antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: X; NC_000086.7 (57212143..57224042). Location: X A5; X 31.21 cM
3D Structure
ModBase 3D Structure for P27548
Purity/Purification
Protein A/G affinity chromatography
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a Armenian Hamster) immunized with mouse Th1 cells. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.
Reconstitution
Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml.
Reconstitution buffer
PBS (sterile)
Preparation and Storage
Stability: Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
Ships Room Temperature or 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-CD40 Ligand antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD40 Ligand antibody
CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site. CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes. Both membrane bound and soluble CD40L induce similar effects on B cells.
Applications Tested/Suitable for anti-CD40 Ligand antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CD40 Ligand antibody
IHC (paraffine): 1:100-1:300
Flow cytometry: 1:100-1:1000
This antibody was selected for its ability to detect mouse CD40 Ligand.
Immunohistochemistry of anti-CD40 Ligand antibody
Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to
IHC staining of CD40 Ligand using MBS690292 (Left panel Red Pulp, Right panel: White Pulp).
Immunohistochemistry of anti-CD40 Ligand antibody
Formalin fixed and paraffin embedded mouse tumor section was subjected to IHC
staining of CD40 Ligand using MBS690292 (Left panel: x 20; Right panel: x 40).
NCBI/Uniprot data below describe general gene information for CD40 Ligand. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035746.2
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NCBI GenBank Nucleotide #
NM_011616.2
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UniProt Primary Accession #
P27548
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UniProt Related Accession #
P27548[Other Products]
Molecular Weight
29,370 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
Cd40lg??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
??[Similar Products]
NCBI Protein Information
CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
Protein Family
CD40 ligand
UniProt Gene Name
Cd40lg??[Similar Products]
UniProt Synonym Gene Names
Cd40l; Tnfsf5; CD40-L; TRAP??[Similar Products]
UniProt Entry Name
CD40L_MOUSE
UniProt Comments for CD40 Ligand
Function: Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching
By similarity.
Subunit structure: Homotrimer.
Subcellular location: Cell membrane; Single-pass type II membrane protein. CD40 ligand, soluble form: Secreted.
Tissue specificity: Specifically expressed on activated CD4+ T-lymphocytes.
Post-translational modification: The soluble form derives from the membrane form by proteolytic processing
By similarity.
Sequence similarities: Belongs to the tumor necrosis factor family.
Research Articles on CD40 Ligand
1. in vivo biochemical or genetic inhibition of NFATc2 activity in megakaryocyte diminishes platelet CD40L implicates the NFATc2/EGR-1 axis as a key regulatory pathway of inflammatory and immunomodulatory activity in platelets
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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