For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinant Porcine CMLC-1. No significant cross-reactivity or interference between Porcine CMLC-1 and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CMLC-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2508978 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CMLC-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CMLC-1. The ELISA analytical biochemical technique of the MBS2508978 kit is based on CMLC-1 antibody-CMLC-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CMLC-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CMLC-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine CMLC-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CMLC-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CMLC-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CMLC-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CMLC-1. You can calculate the concentration of CMLC-1 in the samples by comparing the OD of the samples to the standard curve.

21,932 Da

myosin, light chain 3, alkali; ventricular, skeletal, slow

myosin light chain 3; CMLC1; cardiac myosin light chain 1; myosin light chain 1, slow-twitch muscle B/ventricular isoform; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow; ventricular/slow twitch myosin alkali light chain

Myosin light chain 3

CMLC1; MLC1SB??[Similar Products]

MYL3_HUMAN

MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

MYL3: Regulatory light chain of myosin. Does not bind calcium. Defects in MYL3 are the cause of familial hypertrophic cardiomyopathy type 8 (CMH8). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: I band; sarcomere; muscle myosin complex; cytosol; A band

Molecular Function: actin monomer binding; structural constituent of muscle; motor activity; myosin II heavy chain binding; calcium ion binding

Biological Process: skeletal muscle development; metabolic process; positive regulation of ATPase activity; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Hypertrophic, 8

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