CITED2; ASD8; MRG1; VSD2; MRG-1; P35SRJ; MSG-related protein 1; Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Multiple isoforms of CITED2 are known to exist.

CITED2 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

CITED2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-CITED2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CITED2 Antibody: CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of ***** hematopoietic stem cells (HSCs), with conditional deletion of CITED2 in the ***** mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

CITED2 antibody can be used for detection of CITED2 by Western blot at 1 - 2 mug/mL. For immunofluorescence start at 20 mug/mL.

Western blot analysis of CITED2 in Jurkat cell lysate with CITED2 antibody at (A) 1 and (B) 2 μg/mL.

Immunofluorescence of CITED2 in Jurkat cells with CITED2 antibody at 20 μg/mL.

30 kDa

Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

cbp/p300-interacting transactivator 2; MSG1-related gene 1; MSG-related protein 1; melanocyte-specific gene 1-related gene 1

Cbp/p300-interacting transactivator 2

MRG1; MRG-1??[Similar Products]

CITE2_HUMAN

The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of hypoxia-inducible factor 1-alpha to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

CITED2: Transcriptional coactivator of the p300/CBP-mediated trancription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator- activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left- right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region. Defects in CITED2 are a cause of ventricular septal defect type 2 (VSD2). VSD2 is a common form of congenital cardiovascular anomaly that may occur alone or in combination with other cardiac malformations. It can affect any portion of the ventricular septum, resulting in abnormal communications between the two lower chambers of the heart. Classification is based on location of the communication, such as perimembranous, inlet, outlet (infundibular), central muscular, marginal muscular, or apical muscular defect. Large defects that go unrepaired may give rise to cardiac enlargement, congestive heart failure, pulmonary hypertension, Eisenmenger's syndrome, delayed fetal brain development, arrhythmias, and even sudden cardiac death. Defects in CITED2 are a cause of atrial septal defect type 8 (ASD8). ASD8 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Belongs to the CITED family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cell cycle regulation; Apoptosis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: nuclear chromatin; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; transcription coactivator activity; LBD domain binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: spleen development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; heart development; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell cycle; negative regulation of transcription from RNA polymerase II promoter; liver development; sex determination; cell proliferation; response to estrogen stimulus; positive regulation of cell-cell adhesion; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell differentiation; determination of left/right symmetry; negative regulation of cell migration; negative regulation of apoptosis

Disease: Atrial Septal Defect 8; Ventricular Septal Defect 2

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