Cardiac myosin light chain 1; CMH8; CMLC1; ELC of myosin; Essential light chain of myosin; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B/ventricular isoform; Myosin light chain 1 slow, B; Myosin light chain 3; Myosin, light chain 1, ventricular; myosin, light chain 3, alkali; ventricular, skeletal, slow; myosin, light polypeptide 3, alkali; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow; OTTHUMP00000165922; Slow skeletal ventricular myosin alkali light chain 3; slow-twitch muscle B/ventricular isoform; Ventricular/slow twitch myosin alkali light chain; VLC1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

Mouse

MYL3 antibody detects endogenous levels of total MYL3

Affinity-Chromatography

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-MYL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
Function: Regulatory light chain of myosin. Does not bind calcium.
Subunit Structure: Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational Modifications: The N-terminus is blocked. N-terminus is methylated by METTL11A/NTM1.

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF/ICC: 1:100-1:500

Figure 1: Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissues lysate.

Observed: 22 kDa
Predicted: 22 kDa

myosin light chain 3

myosin light chain 3

Myosin light chain 3

CMLC11 PublicationManual assertion based on opinion iniRef.5; MLC1SB; VLCl1 PublicationManual assertion based on opinion iniRef.2; MLC-lV/sb1 PublicationManual assertion based on opinion iniRef.4??[Similar Products]

MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Regulatory light chain of myosin. Does not bind calcium.

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