Anti -Natural Resistance Associated Macrophage Protein (Nramp1)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000067.5 (74421777..74432625). Location: 1 C3; 1 39.2 cM

Polyclonal

IgG

Rabbit

Recognizes NRAMP1. Immunizing sequence is 100% conserved in red deer, sheep, 93% in bovine, water buffalo, bison and 76% in mouse NRAMP1. No significant sequence homology exist with NRAMP2.

Serum
Serum

Supplied as a liquid, neat serum, 40% glycerol.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-Slc11a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human NRAMP1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. NRAMP1 is expressed in the lysosomal compartment of monocytes and macrophages, lungs, and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. NRAMP1 (human 550 aa, chromosome 2q35; mouse 548 aa) protein is highly hydrophobic integral membrane glycoproteins composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N and C-termini are predicted to be cytoplasmic.

Antibodies; Abs to Ion Transport

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA, Western Blot.
Dilution: Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.

59,741 Da[Similar Products]

solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1

natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh

Natural resistance-associated macrophage protein 1

Bcg; Ity; Lsh; Nramp1??[Similar Products]

NRAM1_MOUSE

SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Vesicle; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass; Cell surface; Transporter

Cellular Component: phagocytic vesicle membrane; cell surface; membrane; lysosome; late endosome; plasma membrane; integral to membrane; endosome membrane; vacuole

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; transporter activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; wound healing; positive regulation of T-helper 1 type immune response; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; antigen processing and presentation of peptide antigen; vacuolar acidification; nitrite transport; T cell cytokine production; transport; T cell proliferation during immune response; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; positive regulation of cytokine production; mRNA stabilization; MAPKKK cascade; L-arginine import; defense response to protozoan; interleukin-2 production; iron ion transport; phagocytosis; MHC class II biosynthetic process; positive regulation of interferon-gamma production; iron ion homeostasis; cellular cadmium ion homeostasis; macrophage activation; response to bacterium; protein import into nucleus, translocation; defense response to bacterium; negative regulation of cytokine production; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; ion transport

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