Anti -Natural Resistance Associated Macrophage Protein (Nramp2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000067.5 (74421777..74432625). Location: 1 C3; 1 39.2 cM

Polyclonal

IgG

Rabbit

Recognizes rat Natural Resistance Associated Macrophage Protein (Nramp2).

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, 0.1% BSA.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?

Small volumes of anti-Slc11a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Natural resistance to infection with unrelated intracellular parasite like Mycobacteria, Salmonella orLeishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed Nramp2, has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike Nramp1, Nramp2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. Nramp2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.

Antibodies; Abs to Ion Transport

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml using control peptide (N0020-01E) to coat plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL.

59,741 Da[Similar Products]

solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1

natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh

Natural resistance-associated macrophage protein 1

Bcg; Ity; Lsh; Nramp1??[Similar Products]

NRAM1_MOUSE

SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Cell surface; Transporter, SLC family; Vesicle; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Cellular Component: phagocytic vesicle membrane; cell surface; membrane; lysosome; late endosome; integral to membrane; plasma membrane; vacuole; endosome membrane

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; transporter activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; positive regulation of T-helper 1 type immune response; wound healing; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; vacuolar acidification; antigen processing and presentation of peptide antigen; nitrite transport; T cell cytokine production; transport; T cell proliferation during immune response; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; positive regulation of cytokine production; mRNA stabilization; MAPKKK cascade; L-arginine import; defense response to protozoan; interleukin-2 production; iron ion transport; phagocytosis; iron ion homeostasis; cellular cadmium ion homeostasis; MHC class II biosynthetic process; macrophage activation; positive regulation of interferon-gamma production; response to bacterium; protein import into nucleus, translocation; defense response to bacterium; negative regulation of cytokine production; activation of protein kinase activity; ion transport; positive regulation of transcription from RNA polymerase II promoter

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