For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of SLC11A1. No significant cross-reactivity or interference between SLC11A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC11A1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SLC11A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7206344 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Natural resistance associated macrophage protein 1 (SLC11A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC11A1. The ELISA analytical biochemical technique of the MBS7206344 kit is based on SLC11A1 antibody-SLC11A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC11A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC11A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Priciple of the assay: SLC11A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLC11A1 antibody and an SLC11A1-HRP conjugate. The assay sample and buffer are incubated together with SLC11A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC11A1 concentration since SLC11A1 from samples and SLC11A1-HRP conjugate compete for the anti-SLC11A1 antibody . Since the number of sites is limited, as more sites are occupied by SLC11A1 from the sample, fewer sites are left to bind SLC11A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC11A1 concentration in each sample is interpolated from this standard curve.

Signal Transduction

59,012 Da

natural resistance-associated macrophage protein 1; NRAMP 1; solute carrier family 11 member 1

Natural resistance-associated macrophage protein 1

NRAMP1; NRAMP 1??[Similar Products]

NRAM1_PIG

Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes ().

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