Product Name
SLC6A19, siRNA
Full Product Name
SLC6A19 siRNA (Mouse)
Product Synonym Names
B0AT1; Sodium-dependent neutral amino acid transporter B(0)AT1; Solute carrier family 6 member 19; System B(0) neutral amino acid transporter AT1
Product Gene Name
SLC6A19 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9D687
Specificity
SLC6A19 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC6A19 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC6A19 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC6A19 sirna
siRNA to inhibit SLC6A19 expression using RNA interference
Applications Tested/Suitable for SLC6A19 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC6A19. It may not necessarily be applicable to this product.
NCBI Accession #
NP_083154.1
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NCBI GenBank Nucleotide #
NM_028878.3
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UniProt Primary Accession #
Q9D687
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UniProt Related Accession #
Q9D687[Other Products]
Molecular Weight
71,367 Da
NCBI Official Full Name
sodium-dependent neutral amino acid transporter B(0)AT1
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter), member 19
NCBI Official Symbol
Slc6a19??[Similar Products]
NCBI Official Synonym Symbols
B<0>AT1; 4632401C08Rik
??[AT1+or+4632401C08Rik" title="Find similar products with B<0>AT1; 4632401C08Rik">Similar Products]
NCBI Protein Information
sodium-dependent neutral amino acid transporter B(0)AT1
UniProt Protein Name
Sodium-dependent neutral amino acid transporter B(0)AT1
UniProt Synonym Protein Names
Solute carrier family 6 member 19; System B(0) neutral amino acid transporter AT1
Protein Family
Sodium-dependent neutral amino acid transporter
UniProt Gene Name
Slc6a19??[Similar Products]
UniProt Synonym Gene Names
B0at1??[Similar Products]
UniProt Entry Name
S6A19_MOUSE
UniProt Comments for SLC6A19
SLC6A19: Transporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium- dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent. Defects in SLC6A19 are a cause of Hartnup disorder (HND). HND is an autosomal recessive abnormality of renal and gastrointestinal neutral amino acid transport noted for its clinical variability. First described in 1956, HND is characterized by increases in the urinary and intestinal excretion of neutral amino acids. Individuals with typical Hartnup aminoaciduria may be asymptomatic, some develop a photosensitive pellagra-like rash, attacks of cerebellar ataxia and other neurological or psychiatric features. Although the definition of HND was originally based on clinical and biochemical abnormalities, its marked clinical heterogeneity has led to it being known as a disorder with a consistent pathognomonic neutral hyperaminoaciduria. Defects in SLC6A19 may be a cause of hyperglycinuria (HG). It is a condition characterized by excess of glycine in the urine. In some cases it is associated with renal colic and renal oxalate stones. SLC6A19 deficiency combined with haploinsufficiency of SLC6A20 or partially inactivating mutations in SLC36A2, can be responsible for hyperglycinuria. Defects in SLC6A19 may be a cause of iminoglycinuria (IG). It is a disorder of renal tubular reabsorption of glycine and imino acids (proline and hydroxyproline), marked by excessive levels of all three substances in the urine. SLC6A19 deficiency combined with haploinsufficiency of SLC6A20 or partially inactivating mutations in SLC36A2, can be responsible for iminoglycinuria. Additional polymorphisms and mutations in SLC6A18 can contribute to the IG phenotype in some families. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A19 subfamily.
Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: membrane; brush border membrane; integral to plasma membrane; integral to membrane; plasma membrane
Molecular Function: protein binding; symporter activity; neutral amino acid transmembrane transporter activity; neurotransmitter:sodium symporter activity
Biological Process: neutral amino acid transport; amino acid transport; transport; neurotransmitter transport
Research Articles on SLC6A19
1. Slc6a19 expression in the intestine is regulated at three different levels involving promoter methylation, histone modification, and opposing transcription factors.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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