Product Name
Cardiac Troponin I (TNNI3), Antibody
Full Product Name
Anti-Cardiac Troponin I Antibody
Product Synonym Names
Cardiac troponin I; cTnI; RCM1; TNNC1; TNNI3; troponin I type 3(cardiac); Troponin I; cardiac muscle
Product Gene Name
anti-TNNI3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19429
Species Reactivity
Human, Mouse, Rat
Purity/Purification
>=95% as determined by SDS-PAGE
Protein A+G Purification
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Immunogen
Troponin I type 3(cardiac)
Preparation and Storage
Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-TNNI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TNNI3 antibody
Troponin I(TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I(cTnI) is produced by cardiac muscle. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction(AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes.
Applications Tested/Suitable for anti-TNNI3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-TNNI3 antibody
WB: 1:5000-1:50000
IHC: 1:50-1:500
IF: 1:50-1:500
Immunohistochemistry (IHC) of anti-TNNI3 antibody
Immunohistochemistry of paraffin-embedded human heart tissue slide using (Cardiac Troponin I Antibody) at dilution of 1:600
Testing Data of anti-TNNI3 antibody
Rat heart tissue were subjected to SDS PAGE followed by western blot with (Cardiac Troponin Iantibody) at dilution of 1:10000
NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000354.4
[Other Products]
NCBI GenBank Nucleotide #
NM_000363.4
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UniProt Primary Accession #
P19429
[Other Products]
UniProt Related Accession #
P19429[Other Products]
Molecular Weight
72 kDa [Similar Products]
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I3, cardiac type
NCBI Official Symbol
TNNI3??[Similar Products]
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
??[Similar Products]
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Synonym Protein Names
Cardiac troponin I
UniProt Gene Name
TNNI3??[Similar Products]
UniProt Synonym Gene Names
TNNC1??[Similar Products]
UniProt Entry Name
TNNI3_HUMAN
NCBI Summary for TNNI3
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
UniProt Comments for TNNI3
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: sarcomere; troponin complex; cytosol
Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding
Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction
Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Research Articles on TNNI3
1. Troponin-I mutation is associated with Hypertrophic cardiomyopathy.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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