Full Product Name
CDH3 siRNA (Mouse)
Product Synonym Names
CDHP; Cadherin-3; Placental cadherin; P-cadherin
Product Gene Name
CDH3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10287
Specificity
CDH3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDH3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CDH3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CDH3 sirna
siRNA to inhibit CDH3 expression using RNA interference
Applications Tested/Suitable for CDH3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CDH3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001032898.1
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NCBI GenBank Nucleotide #
NM_001037809.5
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UniProt Primary Accession #
P10287
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UniProt Secondary Accession #
Q61465; Q8BSL6[Other Products]
UniProt Related Accession #
P10287[Other Products]
Molecular Weight
90,612 Da
NCBI Official Full Name
cadherin-3 isoform a preproprotein
NCBI Official Synonym Full Names
cadherin 3
NCBI Official Symbol
Cdh3??[Similar Products]
NCBI Official Synonym Symbols
Cadp; Cdhp; Pcad; AI385538; P-cadherin
??[Similar Products]
NCBI Protein Information
cadherin-3
UniProt Protein Name
Cadherin-3
UniProt Synonym Protein Names
Placental cadherin; P-cadherin
UniProt Gene Name
Cdh3??[Similar Products]
UniProt Synonym Gene Names
Cdhp; P-cadherin??[Similar Products]
UniProt Entry Name
CADH3_MOUSE
NCBI Summary for CDH3
This gene encodes a calcium-dependent cell-cell adhesion protein containing five cadherin domains. The encoded protein plays a role in epithelial outgrowth, such as that which occurs during the development of hair follicles and limb buds. Loss of function of the related gene in humans results in ectodermal dysplasia, ectrodactyly, and macular dystrophy and congential hypotrichosis with juvenile macular dystrophy. This gene is located in the vicinity of similar cadherin genes on chromosome 8. The proprotein is further cleaved into a functional chain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Comments for CDH3
CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Cellular Component: cell-cell adherens junction; membrane; cytoplasm; plasma membrane; integral to membrane
Molecular Function: metal ion binding; calcium ion binding
Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; retinal homeostasis; negative regulation of transforming growth factor-beta2 production; hair cycle process; cell-cell adhesion; negative regulation of catagen; positive regulation of monophenol monooxygenase activity; keratinization; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; homophilic cell adhesion; cell adhesion
Research Articles on CDH3
1. P-cadherin is a direct PAX3-FOXO1A transcriptional target involved in Alveolar rhabdomyosarcoma aggressiveness.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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