Homeobox protein Nkx-25; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; NKX2-5; CSX; NKX25; NKX2E

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This NKX2-5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the Central region of human NKX2-5.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-NKX2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a homeobox-containing transcription
factor. This transcription factor functions in heart formation and
development. Mutations in this gene cause atrial septal defect with
atrioventricular conduction defect, and also tetralogy of Fallot,
which are both heart malformation diseases. Mutations in this gene
can also cause congenital hypothyroidism non-goitrous type 5, a
non-autoimmune condition. Alternative splicing results in multiple
transcript variants.

Cardiovascular; Developmental Biology; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

NKX2-5 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NKX2-5 antibody detected the NKX2-5 protein (arrow).

34918

NK2 homeobox 5

homeobox protein Nkx-2.5

Homeobox protein Nkx-2.5

CSX; NKX2.5; NKX2E??[Similar Products]

NKX25_HUMAN

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: heart morphogenesis; transcription from RNA polymerase II promoter; ventricular cardiac myofibril development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; cardiac muscle cell differentiation; BMP signaling pathway; atrial cardiac muscle cell development; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; regulation of cardiac muscle cell proliferation; hemopoiesis; heart looping; vasculogenesis; cell differentiation; cardiac muscle contraction; spleen development; ***** heart development; pharyngeal system development; sarcomere organization; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; positive regulation of heart contraction; negative regulation of apoptosis

Disease: Ventricular Septal Defect 3; Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Tetralogy Of Fallot; Hypothyroidism, Congenital, Nongoitrous, 5; Hypoplastic Left Heart Syndrome 2

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