Homeobox protein Nkx-25; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Nkx2-5; Csx; Nkx-25; Nkx2e

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-235 amino acids from the Central region of mouse Nkx2-5.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-Nkx2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

Cardiovascular; Immunology

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western blot analysis of lysate from mouse stomach tissue lysate, using Nkx2-5 Antibody (Center). MBS9211231 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

34163

NK2 homeobox 5

homeobox protein Nkx-2.5

Homeobox protein Nkx-2.5

Csx; Nkx-2.5; Nkx2e??[Similar Products]

NKX25_MOUSE

NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; tongue development; ventricular cardiac myofibril development; heart development; positive regulation of transcription, DNA-dependent; multicellular organismal development; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; atrial cardiac muscle cell development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; hemopoiesis; regulation of cardiac muscle cell proliferation; heart looping; cell differentiation; vasculogenesis; cardiac muscle contraction; cardiac muscle development; spleen development; ***** heart development; pharyngeal system development; sarcomere organization; regulation of transcription from RNA polymerase II promoter; heart contraction; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; positive regulation of heart contraction; negative regulation of apoptosis; endoderm development

Lints T.J.,et al.Development 119:419-431(1993).
Lints T.J.,et al.Development 119:969-969(1993).
Searcy R.D.,et al.Development 125:4461-4470(1998).
Komuro I.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8145-8149(1993).
Kim Y.H.,et al.J. Biol. Chem. 273:25875-25879(1998).

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.