Homeobox protein Nkx-25; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Nkx2-5; Csx; Nkx-25; Nkx2e

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This Mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-225 amino acids from the Central region of human Mouse Nkx2-5.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-Nkx2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

Crown Antibodies; Cardiovascular; Developmental Biology; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western blot analysis of lysates from mouse stomach, rat heart tissue (from left to right), using Mouse Nkx2-5 Antibody (Center). MBS9209264 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

34163

NK2 homeobox 5

homeobox protein Nkx-2.5

Homeobox protein Nkx-2.5

Csx; Nkx-2.5; Nkx2e??[Similar Products]

NKX25_MOUSE

NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: heart morphogenesis; transcription from RNA polymerase II promoter; tongue development; ventricular cardiac myofibril development; heart development; positive regulation of transcription, DNA-dependent; multicellular organismal development; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; cardiac muscle cell differentiation; BMP signaling pathway; regulation of transcription, DNA-dependent; atrial cardiac muscle cell development; cardiac muscle morphogensis; positive regulation of cell proliferation; thyroid gland development; hemopoiesis; regulation of cardiac muscle cell proliferation; heart looping; cell differentiation; vasculogenesis; cardiac muscle contraction; cardiac muscle development; spleen development; ***** heart development; pharyngeal system development; sarcomere organization; regulation of transcription from RNA polymerase II promoter; heart contraction; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; positive regulation of heart contraction; negative regulation of apoptosis; endoderm development

Lints T.J.,et al.Development 119:419-431(1993).
Lints T.J.,et al.Development 119:969-969(1993).
Searcy R.D.,et al.Development 125:4461-4470(1998).
Komuro I.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8145-8149(1993).
Kim Y.H.,et al.J. Biol. Chem. 273:25875-25879(1998).

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