Full Product Name
T TNNI3 (Phospho-Thr142) Antibody
Product Synonym Names
TNNC1; troponin I; cardiac muscle
Product Gene Name
anti-T TNNI3 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19429
Species Reactivity
Human, Mouse, Rat
Specificity
TNNI3 (Phospho-Thr142) antibody detects endogenous levels of TNNI3 only when phosphorylated at threonine 142.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human TNNI3 around the phosphorylation site of threonine 142 (R-P-TP-L-R).
All Sites
Human: Thr142
Mouse: Thr143
Rat: Thr143
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-T TNNI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-T TNNI3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-T TNNI3 antibody
WB: 1:500-1:1000
ELISA: 1:10000
Western Blot (WB) of anti-T TNNI3 antibody
Western blot analysis of extracts from mouse heart, using TNNI3 (Phospho-Thr142) antibody.
NCBI/Uniprot data below describe general gene information for T TNNI3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000354.4
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NCBI GenBank Nucleotide #
NM_000363.4
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UniProt Primary Accession #
P19429
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UniProt Related Accession #
P19429[Other Products]
Molecular Weight
24,008 Da
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I3, cardiac type
NCBI Official Symbol
TNNI3??[Similar Products]
NCBI Official Synonym Symbols
CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
??[Similar Products]
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
UniProt Synonym Protein Names
Cardiac troponin I
UniProt Gene Name
TNNI3??[Similar Products]
UniProt Synonym Gene Names
TNNC1??[Similar Products]
NCBI Summary for T TNNI3
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
UniProt Comments for T TNNI3
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Product References and Citations for anti-T TNNI3 antibody
Raffaella Di Lisi, J. Biol. Chem., Sep 1998; 273: 25371. Emile Missov, Circulation, Nov 1997; 96: 2953-2958. Brandon J. Biesiadecki, J. Biol. Chem., Apr 2004; 279: 13825-13832.
Research Articles on T TNNI3
1. Reversible Covalent Reaction of Levosimendan with Cardiac Troponin C in Vitro and in Situ.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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