For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of TNI. No significant cross-reactivity or interference between TNI and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between TNI and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of TNI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2516066 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNI, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNI. The ELISA analytical biochemical technique of the MBS2516066 kit is based on TNI antibody-TNI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNI in serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TNI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNI. You can calculate the concentration of TNI in the samples by comparing the OD of the samples to the standard curve.

24,160 Da

troponin I type 3 (cardiac)

troponin I, cardiac muscle; cardiac troponin I; troponin 1, type 3

Troponin I, cardiac muscle

Ctni; Tni??[Similar Products]

TNNI3_RAT

cardiac isoform of troponin I, which is a subunit of the troponin complex; inhibits actomyosin ATPase activity in muscle conraction [RGD, Feb 2006]

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding

Cellular Component: sarcomere; troponin complex; myofibril; contractile fiber; cell; cytoplasm

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; striated muscle contraction; regulation of muscle contraction; heart contraction; regulation of systemic arterial blood pressure by ischemic conditions; heart development; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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