Product Name
TCN2, Polyclonal Antibody
Popular Item
Full Product Name
TCN2 Polyclonal Antibody
Product Synonym Names
II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
Product Gene Name
anti-TCN2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20062
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human TCN2
Calculated Molecular Weight
47kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-TCN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCN2 antibody
This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-TCN2 antibody
Polyclonal
Applications Tested/Suitable for anti-TCN2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-TCN2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Western Blot (WB) of anti-TCN2 antibody
Western blot analysis of extracts of various cell lines, using TCN2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
NCBI/Uniprot data below describe general gene information for TCN2. It may not necessarily be applicable to this product.
NCBI Accession #
P20062.3
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UniProt Primary Accession #
P20062
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UniProt Secondary Accession #
Q96FD4; Q9BVI8; Q9UCI5; Q9UCI6; Q9UDM0[Other Products]
UniProt Related Accession #
P20062; AAB25526[Other Products]
NCBI Official Full Name
Transcobalamin-2
NCBI Official Synonym Full Names
transcobalamin II
NCBI Official Symbol
TCN2??[Similar Products]
NCBI Official Synonym Symbols
II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
??[Similar Products]
NCBI Protein Information
transcobalamin-2; macrocytic anemia; vitamin B12-binding protein 2; transcobalamin II; macrocytic anemia
UniProt Protein Name
Transcobalamin-2
UniProt Synonym Protein Names
Transcobalamin II; TC II; TCII
Protein Family
Transcobalamin
UniProt Gene Name
TCN2??[Similar Products]
UniProt Synonym Gene Names
TC2; TC-2; TC II; TCII??[Similar Products]
UniProt Entry Name
TCO2_HUMAN
NCBI Summary for TCN2
This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Comments for TCN2
TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: lysosomal lumen; extracellular space; extracellular region; endosome
Molecular Function: metal ion binding; cobalamin binding
Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process
Disease: Transcobalamin Ii Deficiency
Research Articles on TCN2
1. neither MTHFD G1958A nor TC C776G polymorphisms are an independent risk factor for Down syndrome. However, the combined MTHFD/MTHFR, TC/MTHFR genotypes play a role in the risk of bearing a Down syndrome child in the Chinese population.
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