Product Name
TCN2 / Transcobalamin-II, Recombinant Protein
Full Product Name
Recombinant Human TCN2 / Transcobalamin-II Protein (His tag)
Product Synonym Names
D22S676, D22S750, TC2, TCN2
Product Gene Name
TCN2 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20062
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Predicted N Terminal
Glu 19
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of TCN2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TCN2 recombinant protein
Background: Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.
Description: A DNA sequence encoding the human TCN2 (NP_000346.2) (Met 1-Trp 427) was expressed with a C-terminal polyhistidine tag.
Application Notes for TCN2 recombinant protein
The secreted recombinant human TCN2 comprises 418 amino acids with a predicted molecular mass of 46.7 kDa. It migrates with the molecular weight of approximately 44 kDa in SDS-PAGE under reducing conditions.
SDS-PAGE of TCN2 recombinant protein
NCBI/Uniprot data below describe general gene information for TCN2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000346.2
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NCBI GenBank Nucleotide #
NM_000355.3
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UniProt Primary Accession #
P20062
[Other Products]
UniProt Secondary Accession #
Q96FD4; Q9BVI8; Q9UCI5; Q9UCI6; Q9UDM0[Other Products]
UniProt Related Accession #
P20062; AAB25526[Other Products]
Molecular Weight
44,421 Da
NCBI Official Full Name
transcobalamin-2 isoform 1
NCBI Official Synonym Full Names
transcobalamin 2
NCBI Official Symbol
TCN2??[Similar Products]
NCBI Official Synonym Symbols
II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
??[Similar Products]
NCBI Protein Information
transcobalamin-2
UniProt Protein Name
Transcobalamin-2
UniProt Synonym Protein Names
Transcobalamin II; TC II; TCII
Protein Family
Transcobalamin
UniProt Gene Name
TCN2??[Similar Products]
UniProt Synonym Gene Names
TC2; TC-2; TC II; TCII??[Similar Products]
NCBI Summary for TCN2
This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Comments for TCN2
TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: endosome; extracellular region; lysosomal lumen
Molecular Function: cobalamin binding
Biological Process: cobalamin metabolic process
Disease: Transcobalamin Ii Deficiency
Research Articles on TCN2
1. T. (Q36X).">4 patients with transcobalamin II deficiency were found to have novel mutations, of whom 2 had the same large deletion (homozygous c.1106+1516-1222+1231del). One had c.1107- 347_1222+981delin 364. Another had homozygous c.106C>T. (Q36X).
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