Mouse Cytochrome b-245 heavy chain (CYBB) ELISA kit; CGD; GP91-1; GP91-PHOX; GP91PHOX; NOX2; p91-PHOX; NADPH oxidase 2; cytochrome b-245 beta polypeptide; cytochrome b-245; beta polypeptide

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse CYBB. No significant cross-reactivity or interference between mouse CYBB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CYBB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS937644 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome b-245, beta polypeptide (CYBB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYBB. The ELISA analytical biochemical technique of the MBS937644 kit is based on CYBB antibody-CYBB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYBB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYBB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYBB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYBB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYBB bound in the initial step. The color development is stopped and the intensity of the color is measured.

65,305 Da

cytochrome b-245, beta polypeptide

cytochrome b-245 heavy chain; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox

Cytochrome b-245 heavy chain

Cgd; Cytochrome b558 subunit beta??[Similar Products]

CY24B_MOUSE

This gene encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens. However, increased activity of the encoded protein also leads to the generation of reactive oxygen species that result in oxidative stress and can cause tissue damage. Conversely, loss of function of the related gene in human causes chronic granulomatous disease. Alternative splicing results in multiple transcript variants, although the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.

Protein type: EC 1.-.-.-; Oxidoreductase; Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; mitochondrion; membrane; cell soma; rough endoplasmic reticulum; integral to plasma membrane; endoplasmic reticulum; cytoplasm; dendrite; integral to membrane; plasma membrane; intracellular; NADPH oxidase complex

Molecular Function: N-ethylmaleimide reductase activity; protein binding; FAD binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; malolactic enzyme activity; heme binding; voltage-gated ion channel activity; oxidoreductase activity; sulfur oxygenase reductase activity; F420H2 dehydrogenase activity

Biological Process: respiratory burst; response to drug; transport; superoxide metabolic process; innate immune response; ion transport; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process

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