Full Product Name
CD46 siRNA (Mouse)
Product Synonym Names
MCP; Membrane cofactor protein; CD antigen CD46
Product Gene Name
CD46 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O88174
Specificity
CD46 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CD46 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CD46 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD46 sirna
siRNA to inhibit CD46 expression using RNA interference
Applications Tested/Suitable for CD46 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CD46. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034908.1
[Other Products]
NCBI GenBank Nucleotide #
NM_010778.4
[Other Products]
UniProt Primary Accession #
O88174
[Other Products]
UniProt Secondary Accession #
Q9R0R9[Other Products]
UniProt Related Accession #
O88174[Other Products]
Molecular Weight
33,495 Da
NCBI Official Full Name
membrane cofactor protein
NCBI Official Synonym Full Names
CD46 antigen, complement regulatory protein
NCBI Official Symbol
Cd46??[Similar Products]
NCBI Official Synonym Symbols
Mcp
??[Similar Products]
NCBI Protein Information
membrane cofactor protein
UniProt Protein Name
Membrane cofactor protein
UniProt Synonym Protein Names
CD_antigen: CD46
Protein Family
Membrane cofactor protein
UniProt Gene Name
Cd46??[Similar Products]
UniProt Synonym Gene Names
Mcp??[Similar Products]
UniProt Entry Name
MCP_MOUSE
UniProt Comments for CD46
CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Cell surface; Membrane protein, integral
Cellular Component: Golgi apparatus; inner acrosomal membrane; cell surface; focal adhesion; membrane; basolateral plasma membrane; cytoplasm; extracellular region; acrosome; integral to membrane; cytoplasmic vesicle
Molecular Function: enzyme inhibitor activity; complement binding; cadherin binding; endopeptidase activity
Biological Process: positive regulation of memory T cell differentiation; positive regulation of regulatory T cell differentiation; regulation of Notch signaling pathway; negative regulation of complement activation; single fertilization; T cell mediated immunity; negative regulation of catalytic activity; interleukin-10 production; positive regulation of T cell proliferation; proteolysis; positive regulation of interleukin-10 production
Research Articles on CD46
1. Our findings provide evidence for expression of CD46 in the mouse eye and a role for CD46 in protection against laser-induced choroidal neovascularization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
