Full Product Name
G6PC siRNA (Mouse)
Product Synonym Names
G6PT; Glucose-6-phosphatase; G-6-Pase; G6Pase
Product Gene Name
G6PC sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35576
Specificity
G6PC siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse G6PC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of G6PC sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
G6PC sirna
siRNA to inhibit G6PC expression using RNA interference
Applications Tested/Suitable for G6PC sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for G6PC. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032087.2
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NCBI GenBank Nucleotide #
NM_008061.3
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UniProt Primary Accession #
P35576
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UniProt Secondary Accession #
Q91WV3[Other Products]
UniProt Related Accession #
P35576[Other Products]
Molecular Weight
40,473 Da
NCBI Official Full Name
glucose-6-phosphatase
NCBI Official Synonym Full Names
glucose-6-phosphatase, catalytic
NCBI Official Symbol
G6pc??[Similar Products]
NCBI Official Synonym Symbols
G6pt; G6Pase; AW107337; Glc-6-Pase
??[Similar Products]
NCBI Protein Information
glucose-6-phosphatase
UniProt Protein Name
Glucose-6-phosphatase
Protein Family
Glucose-6-phosphatase
UniProt Gene Name
G6pc??[Similar Products]
UniProt Synonym Gene Names
G6pt; G-6-Pase; G6Pase??[Similar Products]
UniProt Entry Name
G6PC_MOUSE
NCBI Summary for G6PC
The enzyme encoded by this gene is a multisubunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for glucose-6-phosphate, inorganic phosphate, and glucose. This gene is one of three glucose-6-phosphatase catalytic-subunit-encoding genes in mouse. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). [provided by RefSeq, Sep 2015]
UniProt Comments for G6PC
G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family.
Protein type: Transporter; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transporter, SLC family; Carbohydrate Metabolism - galactose; EC 3.1.3.9; Endoplasmic reticulum; Membrane protein, multi-pass; Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein); Membrane protein, integral
Cellular Component: intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: hydrolase activity; glucose-6-phosphatase activity; phosphate binding; phosphotransferase activity, alcohol group as acceptor; catalytic activity
Biological Process: steroid metabolic process; glycogen metabolic process; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; lipid homeostasis; glucose homeostasis; phosphate metabolic process; gluconeogenesis; cholesterol homeostasis; urate metabolic process; triacylglycerol metabolic process; dephosphorylation; regulation of gene expression; glucose-6-phosphate transport
Research Articles on G6PC
1. We conclude that G6PD deficiency at the level of the animals in the present study may not be a risk factor for developing CSN-OT, but this remains to be verified for human subjects
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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