A kinase (PRKA) anchor protein 5; A-kinase anchor 5; A-kinase anchor 79 kDa; AKAP 79; AKAP75; AKAP79; cAMP-dependent protein kinase regulatory subunit II high affinity-binding; H21

For Research Use Only. Not for use in diagnostic procedures.

One 96-Well Plate

Store at 4 degree C.

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Small volumes of AKAP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9500726 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AKAP5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKAP5. The ELISA analytical biochemical technique of the MBS9500726 kit is based on AKAP5 antibody-AKAP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKAP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKAP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Based ELISA Kit

47 kDa

A-kinase anchoring protein 5

A-kinase anchor protein 5

A-kinase anchor protein 5

AKAP79; AKAP-5; AKAP 79??[Similar Products]

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]

AKAP5: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C. Interacts with ADCY8, and enhances its phosphorylation at lipid rafts. Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: cytosol; plasma membrane

Molecular Function: adenylate cyclase binding; calmodulin binding; glutamate receptor binding; protein binding; protein kinase A binding; protein phosphatase 2B binding; SH3 domain binding

Biological Process: positive regulation of cAMP biosynthetic process; protein targeting; signal transduction; synaptic transmission

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