Bcl-3

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 19; NC_000019.9 (45251978..45263301). Location: 19q13.1-q13.2

Monoclonal

Hamster

Bcl-3 [Human and mouse Bcl-3]
This antibody recognizes human and mouse Bcl-3.

100 ug IgG in PBS, pH 7.4, purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Small volumes of anti-BCL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The gene encoding Bcl-3 was first identified as the gene altered in some cases of chronic B cell lymphocytic leukemia and was later found to belong to the I kappa B subfamily of inhibitors of NF kappa B transcription factors. Bcl-3 does not have DNA-binding activity, but it interacts with NF kappa B1 and NF kappa B2 which, when bound to DNA, repress gene transcription. Therefore, Bcl-3 may promote gene expression that is repressed by members of the NF kappa B family. Bcl-3 RNA is highly expressed in spleen and liver with lower levels of expression in heart, skeletal muscle, lung, kidney, and testes.

Apoptosis Antibodies

ELISA, Immunoblot

Immunoblotting: use at 2 ug/ml. A band of approx. 46-52 kDa is detected.
ELISA: end-user should determine optimal concentration for each sample.
Positive control: Jurkat T cell lysate.

47,584 Da[Similar Products]

B-cell CLL/lymphoma 3

B-cell lymphoma 3 protein; BCL-3; OTTHUMP00000200151; OTTHUMP00000200152; proto-oncogene BCL3; B-cell leukemia/lymphoma 3; B-cell lymphoma 3-encoded protein; chronic lymphatic leukemia protein

B-cell lymphoma 3 protein

BCL4; D19S37??[Similar Products]

BCL3_HUMAN

This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. [provided by RefSeq]

Function: Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation

By similarity. Ref.4

Subunit structure: Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD

By similarity. Ref.6 Ref.7

Subcellular location: Nucleus. Cytoplasm

By similarity. Cytoplasm ? perinuclear region

By similarity. Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation

Post-translational modification: Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation

By similarity.Activated by phosphorylation.

Involvement in disease: Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.

Sequence similarities: Contains 7 ANK repeats.

Caution: It is uncertain whether Met-1 or Met-9 is the initiator.

Sequence caution: The sequence AAA51815.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA51816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH64993.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Mitchell, TC et al. (2001) Nature Immunology 2: 397-402.
Watanabe, N et al. (1997) EMBO J 16: 3609-20.
Nolan, GP et al. (1993) Molecular and Cellular Biology 13: 3557-66.

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