Anti -Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (19744226..19771116). Location: 22q11.21

Monoclonal

IgG1k

9H202

Mouse

Recognizes human brachyury.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-TBX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Antibodies; Abs to Transcription Factors

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

40,353 Da[Similar Products]

T-box 1

T-box transcription factor TBX1; brachyury; OTTHUMP00000197322; OTTHUMP00000197323; OTTHUMP00000197324; Testis-specific T-box protein; T-box 1 transcription factor C

T-box 1

Q152R5_HUMAN

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

TBX1: Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS). DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life. Defects in TBX1 are a cause of DiGeorge syndrome (DGS). Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS). Defects in TBX1 are a cause of conotruncal heart malformations (CTHM). CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein homodimerization activity; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: heart morphogenesis; retinoic acid receptor signaling pathway; muscle development; heart development; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; cell fate specification; middle ear morphogenesis; ear morphogenesis; anterior/posterior pattern formation; sensory perception of sound; epithelial cell differentiation; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; thyroid gland development; mesoderm development; angiogenesis; neural crest cell migration; muscle morphogenesis; blood vessel development; tongue morphogenesis; inner ear morphogenesis; pharyngeal system development; transcription, DNA-dependent; thymus development; outer ear morphogenesis; semicircular canal morphogenesis; embryonic viscerocranium morphogenesis; social behavior; embryonic cranial skeleton morphogenesis; pattern specification process; parathyroid gland development; odontogenesis of dentine-containing teeth; regulation of transcription from RNA polymerase II promoter; cell proliferation; negative regulation of cell differentiation; artery morphogenesis; blood vessel morphogenesis; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; soft palate development; positive regulation of protein amino acid phosphorylation; lymph vessel development; determination of left/right symmetry; vagus nerve morphogenesis; positive regulation of epithelial cell proliferation

Disease: Conotruncal Heart Malformations; Digeorge Syndrome; Velocardiofacial Syndrome; Tetralogy Of Fallot

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