Anti -CTP Phosphocholine cytidylyltransferase (CCT alpha, CCTa)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_000003.11 (195965253..196014584, complement). Location: 3q29

Polyclonal

IgG

Rabbit

Recognizes human CTP Phosphocholine Cytidylytransferase at 42kD.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA and 50% glycerol.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-PCYT1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTa, CCTB2, and CCTB3 (1,2). CCTa is the major isoform that is expressed in most tissues (3). CCTa is essential in the synthesis and secretion of surfactant by alveolar epithelial cells and is important in maintaining the phosphatidylcholine level that regulates lipoprotein as- sembly and secretion in hepatocytes (4,5). CCTa is a major component in membrane biogenesis during cytokine secretion by stimulated macrophages (6). Monoubiquitination of CCTa prevents it from entering the nucleus and leads to its degradation by lysosome (7).

Antibodies; Abs to Enzymes

Western Blot (WB), Immunoprecipitation (IP). Other applications not tested.

Suitable for use in Western Blot and Immunoprecipitation.
Dilution:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

41,731 Da[Similar Products]

phosphate cytidylyltransferase 1, choline, alpha

choline-phosphate cytidylyltransferase A; CT A; CCT A; CCT-alpha; OTTHUMP00000208442; OTTHUMP00000208443; OTTHUMP00000208444; OTTHUMP00000208447; phosphorylcholine transferase A; CTP:phosphocholine cytidylyltransferase A

Choline-phosphate cytidylyltransferase A

CTPCT; PCYT1??[Similar Products]

PCY1A_HUMAN

This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]

PCYT1A: Controls phosphatidylcholine synthesis. Homodimer. By phosphorylation. Belongs to the cytidylyltransferase family.

Protein type: Transferase; EC 2.7.7.15; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; glycogen granule; cytosol

Molecular Function: choline-phosphate cytidylyltransferase activity; protein homodimerization activity; lipid binding

Biological Process: CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process

Disease: Spondylometaphyseal Dysplasia With Cone-rod Dystrophy

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