Full Product Name
Rabbit anti CD25 Polyclonal Antibody
Product Synonym Names
IL2RA; Interleukin2 receptor alpha
Product Gene Name
anti-CD25 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P25094
Specificity
This antibody reacts with human. The other species are not tested.
Purity/Purification
Purified by Epitope Affinity Purification
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Preparation
Recombinant full length (1-272 aa) of human CD25.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD25 antibody
The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and ***** thymus. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation.
Applications Tested/Suitable for anti-CD25 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-CD25 antibody
ELISA: 0.01-0.1 ug/ml
Flow Cytometry: 5-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.
NCBI Accession #
CAA44297.1
[Other Products]
UniProt Primary Accession #
P25094
[Other Products]
UniProt Related Accession #
P01589[Other Products]
Molecular Weight
17,946 Da
NCBI Official Full Name
CD25
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA??[Similar Products]
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
??[Similar Products]
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Peripheral myelin protein 22
UniProt Synonym Protein Names
Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein; SAG
UniProt Gene Name
Pmp22??[Similar Products]
UniProt Synonym Gene Names
Cd25; Pmp-22; PMP-22; SAG??[Similar Products]
NCBI Summary for CD25
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
UniProt Comments for CD25
Might be involved in growth regulation, and in myelinization in the peripheral nervous system.
Product References and Citations for anti-CD25 antibody
Amu, S. and Brisslert, M. Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin. Dev. Immunol. 2011, 481948 (2011).
Research Articles on CD25
1. The study demonstrated Increased level of CD25 in patients with active vitilligo.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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