For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat ACVR2B. No significant cross-reactivity or interference between Rat ACVR2B and analogues was observed.

Store at 2-8 degree C.

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Small volumes of ACVR2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280140 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activin receptor type-2B (ACVR2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACVR2B. The ELISA analytical biochemical technique of the MBS280140 kit is based on ACVR2B antibody-ACVR2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACVR2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACVR2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACVR2B in samples. An antibody specific for ACVR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACVR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACVR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACVR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

57,857 Da

activin A receptor type 2B

activin receptor type-2B

Activin receptor type-2B

Actriib; ACTR-IIB??[Similar Products]

plays a role in reproductive physiology and endocrinology [RGD, Feb 2006]

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type2 subfamily

Chromosomal Location of Human Ortholog: 8q32

Cellular Component: cytoplasm; integral component of membrane; plasma membrane; protein complex; receptor complex

Molecular Function: activin binding; ATP binding; growth factor binding; metal ion binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; signal transducer, downstream of receptor, with serine/threonine kinase activity; transforming growth factor beta binding; transmembrane receptor protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; activin receptor signaling pathway; anterior/posterior pattern specification; blood vessel remodeling; BMP signaling pathway; determination of left/right symmetry; embryonic foregut morphogenesis; gastrulation with mouth forming second; heart development; insulin secretion; kidney development; lung development; lymphangiogenesis; mesoderm development; negative regulation of transcription from RNA polymerase II promoter; odontogenesis of dentine-containing teeth; organ growth; palate development; pancreas development; pattern specification process; positive regulation of activin receptor signaling pathway; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; post-embryonic development; protein amino acid phosphorylation; regulation of signal transduction; regulation of transcription, DNA-templated; response to glucose stimulus; sexual reproduction; signal transduction; skeletal morphogenesis; skeletal system development; transmembrane receptor protein serine/threonine kinase signaling pathway

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