PINK1; BRPK; PARK6BRPK; PTEN induced putative kinase 1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

PINK1 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will only detect the longer isoform. PINK1 antibody will detect the cleaved and uncleaved form of PINK1.

PINK1 antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

PINK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Small volumes of anti-PINK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1). PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2). PINK1 is cleaved by the mitochondrial protease PARL (3). Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4).

ELISA (EIA), Western Blot (WB)

PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 mug/ml.

Western blot analysis of PINK1 in A431 cell lysate with PINK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of PINK1 in mouse brain tissue with PINK1 Antibody at 5 μg/mL.

Immunofluorescence of PINK1 in mouse brain tissue with PINK1 Antibody at 20 μg/mL.

Green: PINK1 antibody (7859)
Red: Phylloidin staining
Blue: DAPI staining

Western blot analysis of PINK1 in (A) mouse and (B) rat spleen tissue lysate with PINK1 antibody at 1 μg/mL.

Predicted: 55 kDa

Observed: 53 kDa

PTEN induced putative kinase 1

serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1

Serine/threonine-protein kinase PINK1, mitochondrial

PINK1_HUMAN

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Mitochondrial; Membrane protein, integral; Protein kinase, Other; EC 2.7.11.1; Other group; NKF2 family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrial outer membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; axon; mitochondrial inner membrane; cytoplasm; TORC2 complex; mitochondrial intermembrane space; cytosol; chromatin; nucleus; integral to mitochondrial outer membrane

Molecular Function: protein kinase B binding; protein serine/threonine kinase activity; protein binding; protease activator activity; protease binding; ubiquitin protein ligase binding; magnesium ion binding; kinase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of dopamine secretion; negative regulation of JNK cascade; positive regulation of translation; regulation of protein ubiquitination; protein ubiquitination; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of macroautophagy; regulation of mitochondrial membrane potential; positive regulation of ubiquitin-protein ligase activity; response to stress; regulation of protein complex assembly; negative regulation of neuron apoptosis; regulation of hydrogen peroxide metabolic process; mitochondrion organization and biogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; activation of protein kinase B; positive regulation of synaptic transmission, dopaminergic; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mitochondrion degradation; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; response to oxidative stress; phosphorylation

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset

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