Product Name
TGFBI, siRNA
Full Product Name
TGFBI siRNA (Mouse)
Product Synonym Names
Transforming growth factor-beta-induced protein ig-h3; Beta ig-h3
Product Gene Name
TGFBI sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P82198
Specificity
TGFBI siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TGFBI gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TGFBI sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TGFBI sirna
siRNA to inhibit TGFBI expression using RNA interference
Applications Tested/Suitable for TGFBI sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TGFBI. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033395.1
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NCBI GenBank Nucleotide #
NM_009369.4
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UniProt Primary Accession #
P82198
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UniProt Secondary Accession #
Q3U9R1[Other Products]
UniProt Related Accession #
P82198[Other Products]
Molecular Weight
74,597 Da
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor, beta induced
NCBI Official Symbol
Tgfbi??[Similar Products]
NCBI Official Synonym Symbols
68kDa; big-h3; Beta-ig; AI181842; AI747162
??[Similar Products]
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
Protein Family
Transforming growth factor-beta-induced protein
UniProt Gene Name
Tgfbi??[Similar Products]
UniProt Synonym Gene Names
Beta ig-h3??[Similar Products]
UniProt Entry Name
BGH3_MOUSE
UniProt Comments for TGFBI
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Extracellular matrix; Secreted, signal peptide; Cell adhesion; Secreted
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane; trans-Golgi network
Molecular Function: collagen binding; extracellular matrix binding; cell adhesion molecule binding
Biological Process: extracellular matrix organization and biogenesis; cell adhesion
Research Articles on TGFBI
1. The results support a novel inflammatory pathway for diabetic retinopathy. This pathway is initiated by TGFbeta released from macrophages, which promotes synthesis and release of BIGH3 protein by REC and REC apoptosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
