Product Name
TGF beta, Polyclonal Antibody
Full Product Name
TGF beta antibody
Product Synonym Names
Polyclonal TGF beta; Anti-TGF beta; TGFB1; Transforming Growth Factor-beta1; TGF beta-1; TGF beta 1; TGF-1b; Differentiation inhibiting factor; Cartilage-inducing factor
Product Gene Name
anti-TGF beta antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Specificity
Human LAP derived TGF beta 1 precursor
Form/Format
Lyophilized from a 0.2 um filtered solution in PBS and NaCl with Trehalose.
Biological Significance
Transforming growth factor (sometimes referred to as Tumor growth factor, or TGF) is used to describe two classes of polypeptide growth factors, TGF alpha and TGFBeta.
Bioactivity
ND50 is typically 15-30 ug/ml in the presence of 125 ng/ml of?recombinant human LAP TGF beta 1 and 0.25 ng/ml TGF beta 1.
Endotoxin Levels
<10 ng per 1 mg of the antibody as determined by the LAL method.
Immunogen
TGF beta antibody was raised in goat using CHO cell-derived recombinant human LAP (TGF-beta1) as the immunogen.
Preparation and Storage
Store at -20 degree C until reconstitution. Following reconstitution product may be stored at 4 degree C in the short term. For long term storage aliquot and freeze at -20 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-TGF beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TGF beta antibody
Affinity purified Goat polyclonal TGF beta antibody
Product Categories/Family for anti-TGF beta antibody
Cytokines & Growth Factors; Purified Polyclonal Antibodies
Applications Tested/Suitable for anti-TGF beta antibody
ELISA (EIA), Flow Cytometry (FC/FACS),?Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Neutralization
Application Notes for anti-TGF beta antibody
FC: 2.5 ug/10^6 cells
IHC: 5-15 ug/ml
ICC: 5-15 ug/ml
WB: 1 ug/ml
NCBI/Uniprot data below describe general gene information for TGF beta. It may not necessarily be applicable to this product.
NCBI Accession #
ACT75673.1
[Other Products]
UniProt Secondary Accession #
O14471; O14472; O14476; O43216; O43217; O43218; O43219; Q53XM1; D3DQB1[Other Products]
UniProt Related Accession #
Q15582[Other Products]
Molecular Weight
74,681 Da
NCBI Official Full Name
transforming growth factor beta, partial
NCBI Official Synonym Full Names
transforming growth factor, beta-induced, 68kDa
NCBI Official Symbol
TGFBI??[Similar Products]
NCBI Official Synonym Symbols
CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
??[Similar Products]
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
UniProt Synonym Protein Names
Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP
UniProt Gene Name
TGFBI??[Similar Products]
UniProt Synonym Gene Names
BIGH3; Beta ig-h3; RGD-CAP??[Similar Products]
UniProt Entry Name
BGH3_HUMAN
NCBI Summary for TGF beta
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
UniProt Comments for TGF beta
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Extracellular matrix; Secreted; Cell adhesion; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; plasma membrane; extracellular region; basement membrane; trans-Golgi network
Molecular Function: integrin binding; collagen binding; protein binding; extracellular matrix binding
Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; negative regulation of cell adhesion; angiogenesis; cell adhesion
Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Avellino Type; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I
Research Articles on TGF beta
1. Silencing of DDR1 inhibited tumor cell growth and motility, and induced TGFBI expression, both in vitro and in vivo.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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