Interleukin-23 receptor; IL-23 receptor; IL-23R; IL23R

For Research Use Only. Not for use in diagnostic procedures.

Natural and recombinant Human IL-23R

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of IL-23R elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1751134 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-23R, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-23R. The ELISA analytical biochemical technique of the MBS1751134 kit is based on IL-23R antibody-IL-23R antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-23R antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-23R. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: Human IL-23R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A a monoclonal antibody from mouse specific for IL-23R has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: I25-I354) and test samples are added to the wells, a biotinylated detection a biotinylated polyclonal antibody from goat specific for IL-23R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-23R amount of sample captured in plate.

Background: Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand- dependent manner.

Immunology; Cell Type Markers; Cytokines; Immunology; immunoGlobulins; Receptors; Innate Immunity; Interleukins; Signal Transduction; Signaling Pathway; Nuclear Signaling; stats; Epigenetics and Nuclear Signaling; nuclear Signaling Pathways

ELISA (EIA)

25,551 Da

interleukin 23 receptor

interleukin-23 receptor

Interleukin-23 receptor

IL-23 receptor; IL-23R??[Similar Products]

The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq, Jul 2008]

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

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