For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against PINK1. It has been selected for its ability to recognize PINK1 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-PINK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Lane1: Human Serum; Lane2: Mouse Heart Tissue.

Western Blot: Sample: Recombinant PINK1, Human.

DAB staining on IHC-P; Samples: Human Thyroid Cancer Tissue.

DAB staining on IHC-P; Samples: Human Rectum Tissue

DAB staining on IHC-P; Samples: Human Kidney Tissue.

30,104 Da

PTEN induced putative kinase 1

serine/threonine-protein kinase PINK1, mitochondrial

Serine/threonine-protein kinase PINK1, mitochondrial

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Membrane protein, integral; Mitochondrial; NKF2 family; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: axon; cell body; chromatin; cytoplasm; cytoskeleton; cytosol; growth cone; integral to mitochondrial outer membrane; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrial outer membrane translocase complex; mitochondrion; nucleus; perinuclear region of cytoplasm; TORC2 complex; ubiquitin ligase complex

Molecular Function: ATP binding; calcium-dependent protein kinase activity; kinase activity; magnesium ion binding; protease activator activity; protease binding; protein binding; protein kinase activity; protein kinase B binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding

Biological Process: activation of protein kinase B activity; cellular response to hydrogen sulfide; cellular response to hypoxia; cellular response to oxidative stress; establishment of protein localization to mitochondrion; macroautophagy; maintenance of protein location in mitochondrion; mitochondrion degradation; mitochondrion organization; mitochondrion to lysosome transport; negative regulation of gene expression; negative regulation of JNK cascade; negative regulation of macroautophagy; negative regulation of mitochondrial fission; negative regulation of neuron apoptosis; negative regulation of reactive oxygen species metabolic process; peptidyl-serine phosphorylation; positive regulation of ATP biosynthetic process; positive regulation of cristae formation; positive regulation of dopamine secretion; positive regulation of free ubiquitin chain polymerization; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of macroautophagy; positive regulation of mitochondrial fission; positive regulation of mitophagy in response to mitochondrial depolarization; positive regulation of peptidase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein dephosphorylation; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of protein targeting to mitochondrion; positive regulation of protein ubiquitination; positive regulation of release of cytochrome c from mitochondria; positive regulation of synaptic transmission, dopaminergic; positive regulation of transcription factor activity; positive regulation of translation; positive regulation of ubiquitin-protein transferase activity; protein amino acid phosphorylation; protein stabilization; protein ubiquitination; regulation of cellular response to oxidative stress; regulation of hydrogen peroxide metabolic process; regulation of mitochondrial membrane potential; regulation of mitochondrion organization; regulation of oxidative phosphorylation; regulation of protein complex assembly; regulation of protein ubiquitination; regulation of reactive oxygen species metabolic process; regulation of synaptic vesicle transport; response to oxidative stress; response to stress; TORC2 signaling; ubiquitin-dependent protein catabolic process

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset

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