Rabbit anti-PCSK9; Proprotein convertase subtilisin/kexin type 9 (PCSK9) Proprotein concertase PC9; Subtilisin/Kexin-like protease PC9; FH3; NARC1

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (55505149..55530526). Location: 1p32.3

Rabbit

This antibody recognizes ~74 kDa of human PCSK9 protein. It also reacts with mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Epitope-Specific Affinity Purification.

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul (lot specific)

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Small volumes of anti-PCSK9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a member of the serine protease family with a critical role in cholesterol metabolism by controlling the levels of low density lipoprotein (LDL) particles that circulate in the bloodstream. Mutation in the PCSK9 gene is associated with autosomal dominant hypercholesterolemia which is characterized by an increase in LDL cholesterol levels. PCSK9 is expressed in various tissues such as liver, kidney, lung, spleen, etc. Human PCSK9 precursor is 692 aa protein with 74kDa molecular weight. Its mature form has molecular weight ranging of 62-66 kDa in the tissues.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml

Western Blot: The whole cell lysate derived from HT-29 (lane 1), Mouse Liver (lane 2), mouse lung (lane 3) were immunoblotted by Rabbit anti-PCSK9 (Cat#MBS462174)

74 kDa or 64 kDa

proprotein convertase subtilisin/kexin type 9

proprotein convertase subtilisin/kexin type 9; OTTHUMP00000009833; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein

Proprotein convertase subtilisin/kexin type 9

NARC1??[Similar Products]

PCSK9_HUMAN

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq]

Function: May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.

Cofactor: Calcium

Probable.

Enzyme regulation: Inhibited by EGTA.

Subunit structure: The precursor protein but not the mature protein may form multimers.

Subcellular location: Secreted.

Tissue specificity: Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.

Post-translational modification: The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.

Polymorphism: Variant Leu-23 ins polymorphism in PCSK9 might have a modifier effect on LDLR mutation and familial hypercholesterolemia.

Involvement in disease: Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [

MIM:603776]. FH3 inheritance is autosomal dominant. Ref.13

Sequence similarities: Belongs to the peptidase S8 family.Contains 1 peptidase S8 domain.

Sequence caution: The sequence BAC11572.1 differs from that shown. Reason: Frameshift at position 494.

Lopez D (2008) PCSK9: an enigmatic protease. Biochim Biophys Acta. 1781 (4): 184-91.

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