Full Product Name
ABR siRNA (Mouse)
Product Synonym Names
Active breakpoint cluster region-related protein
Product Gene Name
ABR sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5SSL4
Specificity
ABR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ABR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ABR sirna
siRNA to inhibit ABR expression using RNA interference
Applications Tested/Suitable for ABR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ABR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001278115.1
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NCBI GenBank Nucleotide #
NM_001291186.1
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UniProt Primary Accession #
Q5SSL4
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UniProt Secondary Accession #
Q3U5G0; Q3UQJ6; Q3UY38; Q5SYJ5; Q5SYJ7; Q5SYJ8; Q5SYJ9; Q6PCY1; Q6PDH3[Other Products]
UniProt Related Accession #
Q5SSL4[Other Products]
Molecular Weight
93,773 Da
NCBI Official Full Name
active breakpoint cluster region-related protein isoform 4
NCBI Official Synonym Full Names
active BCR-related gene
NCBI Official Symbol
Abr??[Similar Products]
NCBI Protein Information
active breakpoint cluster region-related protein
UniProt Protein Name
Active breakpoint cluster region-related protein
UniProt Gene Name
Abr??[Similar Products]
UniProt Entry Name
ABR_MOUSE
UniProt Comments for ABR
ABR: GTPase-activating protein for RAC and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); GAPs; GAPs, Rac/Rho; ATYPICAL group; BCR family
Cellular Component: membrane; plasma membrane; intracellular; cytosol
Molecular Function: Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTPase activator activity
Biological Process: inner ear morphogenesis; negative regulation of neutrophil degranulation; negative regulation of inflammatory response; positive regulation of phagocytosis; regulation of Rho protein signal transduction; response to lipopolysaccharide; brain development; neuromuscular process controlling balance; signal transduction; actin cytoskeleton organization and biogenesis; negative regulation of cell migration; positive regulation of GTPase activity
Research Articles on ABR
1. Loss of abr expression causes defects in spine development.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
