Product Name
CDH23, siRNA
Full Product Name
CDH23 siRNA (Human)
Product Synonym Names
KIAA1774; KIAA1812; Cadherin-23; Otocadherin
Product Gene Name
CDH23 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9H251
Specificity
CDH23 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDH23 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CDH23 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CDH23 sirna
siRNA to inhibit CDH23 expression using RNA interference
Applications Tested/Suitable for CDH23 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CDH23. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001165401.1
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NCBI GenBank Nucleotide #
NM_001171930.1
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UniProt Primary Accession #
Q9H251
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UniProt Secondary Accession #
Q5QGS1; Q5QGS2; Q5QGS5; Q5QGS6; Q5XKN2; Q6UWW1; Q96JL3; Q9H4K9; C4IXS9; F6U049[Other Products]
UniProt Related Accession #
Q9H251[Other Products]
Molecular Weight
23,900 Da
NCBI Official Full Name
cadherin-23 isoform 3
NCBI Official Synonym Full Names
cadherin-related 23
NCBI Official Symbol
CDH23??[Similar Products]
NCBI Official Synonym Symbols
USH1D; CDHR23
??[Similar Products]
NCBI Protein Information
cadherin-23
UniProt Protein Name
Cadherin-23
UniProt Synonym Protein Names
Otocadherin
UniProt Gene Name
CDH23??[Similar Products]
UniProt Synonym Gene Names
KIAA1774; KIAA1812??[Similar Products]
UniProt Entry Name
CAD23_HUMAN
NCBI Summary for CDH23
This gene is a member of the cadherin superfamily, whose genes encode calcium dependent cell-cell adhesion glycoproteins. The encoded protein is thought to be involved in stereocilia organization and hair bundle formation. The gene is located in a region containing the human deafness loci DFNB12 and USH1D. Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of this cadherin-like gene. Upregulation of this gene may also be associated with breast cancer. Alternative splice variants encoding different isoforms have been described. [provided by RefSeq, May 2013]
UniProt Comments for CDH23
CDH23: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. CDH23 is required for establishing and/or maintaining the proper organization of the stereocilia bundle of hair cells in the cochlea and the vestibule during late embryonic/early postnatal development. It is part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Defects in CDH23 are the cause of Usher syndrome type 1D (USH1D). USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness. Defects in CDH23 are a cause of Usher syndrome type 1D/F (USH1DF). USH1DF patients are heterozygous for mutations in CDH23 and PCDH15, indicating a digenic inheritance pattern. Defects in CDH23 are the cause of deafness autosomal recessive type 12 (DFNB12). DFNB12 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q22.1
Cellular Component: stereocilium; membrane; plasma membrane; integral to membrane
Molecular Function: protein binding; calcium ion binding
Biological Process: sensory perception of sound; cytosolic calcium ion homeostasis; visual perception; calcium ion transport; sensory perception of light stimulus; photoreceptor cell maintenance; response to stimulus; calcium-dependent cell-cell adhesion; homophilic cell adhesion; equilibrioception
Disease: Deafness, Autosomal Recessive 12; Usher Syndrome, Type Id
Research Articles on CDH23
1. Description of the spectrum of mutations in CDH23 in 374 families with autosomal recessive, non-syndromic hearing loss from India.
Precautions
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Disclaimer
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