Anti -Spectrin, alpha, Erythrocytic 1 (Erythroid alpha-Spectrin, Spectrin alpha Chain Erythrocyte, SPTA, SPTA1, Elliptocytosis 2, EL2, HPP, HS3, SPH3)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (158580496..158656506, complement). Location: 1q21

Monoclonal

IgG1

AF10

Mouse

Recognizes the 240kD human erythroid a-spectrin.

Ascites
Serum

Supplied as a liquid in PBS, 1% BSA, 0.1 % sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?

Small volumes of anti-SPTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics.

Antibodies; Abs to Spectrin

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry: Frozen

280,014 Da[Similar Products]

spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

spectrin alpha chain, erythrocytic 1; alpha-I spectrin; erythroid alpha-spectrin; spectrin alpha chain, erythrocyte

Spectrin alpha chain, erythrocytic 1

SPTA??[Similar Products]

SPTA1_HUMAN

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq, Jul 2008]

SPTA1: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2). EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP). HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells. Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3); also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive. Belongs to the spectrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: spectrin; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding

Biological Process: axon guidance; regulation of cell shape; plasma membrane organization and biogenesis; positive regulation of protein binding; actin filament capping; actin filament organization; hemopoiesis; positive regulation of T cell proliferation; porphyrin biosynthetic process; lymphocyte homeostasis

Disease: Elliptocytosis 2; Pyropoikilocytosis, Hereditary; Spherocytosis, Type 3

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