Full Product Name
Goat Polyclonal to Human TGFBI
Product Synonym Names
Anti-TGFBI Antibody (Internal) IHC-plus; TGFBI; BIGH3; Beta ig-h3; CDG2; CSD; CSD2; CSD3; CDGG1; EBMD; Kerato-epithelin; LCD1; RGD-CAP; CDB1; CSD1; Human TGFBI
Product Gene Name
anti-TGFBI antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15582
Species Reactivity
Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (lot specific)
Immunogen Description
Synthetic peptide C-QLYTDRTEKLRPE from an internal region of human TGFBI (NP_000349.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum (100%).
Immunogen Type
Synthetic peptide
Immunogen
TGFBI antibody was raised against synthetic peptide C-QLYTDRTEKLRPE from an internal region of human TGFBI (NP_000349.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum (100%); Marmoset, Platypus (92%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-TGFBI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-TGFBI antibody
Family: TGF beta
Applications Tested/Suitable for anti-TGFBI antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TGFBI antibody
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.2 - 0.5 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 70 kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 74.7 kDa according to Human NP_000349.1). Recommended concentration: 0.2-0.5 ug/ml.
Immunohistochemistry (IHC) of anti-TGFBI antibody
Anti-TGFBI antibody IHC of human kidney, renal duct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Western Blot (WB) of anti-TGFBI antibody
TGFBI antibody (0.3 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for TGFBI. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000349.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000358.2
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UniProt Primary Accession #
Q15582
[Other Products]
UniProt Secondary Accession #
O14471; O14472; O14476; O43216; O43217; O43218; O43219; Q53XM1; D3DQB1[Other Products]
UniProt Related Accession #
Q15582[Other Products]
Molecular Weight
74,681 Da
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor, beta-induced, 68kDa
NCBI Official Symbol
TGFBI??[Similar Products]
NCBI Official Synonym Symbols
CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
??[Similar Products]
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3; RGD-CAP; beta ig-h3; kerato-epithelin; RGD-containing collagen-associated protein; transforming growth factor, beta-induced, 68kD
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
UniProt Synonym Protein Names
Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP
Protein Family
Transforming growth factor-beta-induced protein
UniProt Gene Name
TGFBI??[Similar Products]
UniProt Synonym Gene Names
BIGH3; Beta ig-h3; RGD-CAP??[Similar Products]
UniProt Entry Name
BGH3_HUMAN
NCBI Summary for TGFBI
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
UniProt Comments for TGFBI
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Secreted, signal peptide; Secreted; Extracellular matrix; Cell adhesion
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; plasma membrane; basement membrane; trans-Golgi network
Molecular Function: integrin binding; collagen binding; protein binding; extracellular matrix binding
Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; angiogenesis; negative regulation of cell adhesion; cell adhesion
Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Avellino Type; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I
Research Articles on TGFBI
1. The H19-miR-675 axis acts as a suppressor of prostate cancer metastasis via modulation of TGFBI expression.
Precautions
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Disclaimer
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