Full Product Name
Anti-SPTA1 Monoclonal Antibody
Product Gene Name
anti-SPTA1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P02549
Specificity
No cross reactivity with other proteins.
Purity/Purification
Affinity-Chromatography
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human SPTA1 Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-SPTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SPTA1 antibody
Rabbit IgG Monoclonal antibody for P02549 detection. Tested positive for WB IHC in Human
Applications Tested/Suitable for anti-SPTA1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SPTA1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
Western Blot (WB) of anti-SPTA1 antibody
Figure 1. Western blot analysis of SPTA1 using anti-SPTA1 antibody (M03376).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTA1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SPTA1.
NCBI/Uniprot data below describe general gene information for SPTA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003117.2
[Other Products]
NCBI GenBank Nucleotide #
NM_003126.3
[Other Products]
UniProt Primary Accession #
P02549
[Other Products]
UniProt Secondary Accession #
Q15514; Q5VYL1; Q5VYL2; Q6LDY5[Other Products]
UniProt Related Accession #
P02549[Other Products]
Molecular Weight
279,674 Da
NCBI Official Full Name
spectrin alpha chain, erythrocytic 1
NCBI Official Synonym Full Names
spectrin alpha, erythrocytic 1
NCBI Official Symbol
SPTA1??[Similar Products]
NCBI Official Synonym Symbols
EL2; HPP; HS3; SPH3; SPTA
??[Similar Products]
NCBI Protein Information
spectrin alpha chain, erythrocytic 1
UniProt Protein Name
Spectrin alpha chain, erythrocytic 1
UniProt Synonym Protein Names
Erythroid alpha-spectrin
UniProt Gene Name
SPTA1??[Similar Products]
UniProt Synonym Gene Names
SPTA??[Similar Products]
NCBI Summary for SPTA1
This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017]
UniProt Comments for SPTA1
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Research Articles on SPTA1
1. a novel HE case with a His54Pro mutation in the SPTA1 gene was reported. The results suggested that the His54Pro mutation influenced the role of erythrocyte membrane proteins without reducing its level of expression.
Precautions
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Disclaimer
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