Purified Rabbit Polyclonal Antibody (Pab); Forkhead box protein H1; Forkhead activin signal transducer 1; Fast-1; Forkhead activin signal transducer 2; Fast-2; Foxh1; Fast1; Fast2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Ig

Rabbit

This Mouse Foxh1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 290-322 amino acids from the C-terminal region of Mouse Foxh1.

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-Foxh1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

Western Blot (WB)

WB ~~ 1:2000

All lanes : Anti-Foxh1 Antibody (C-term) at 1:2000 dilution
Lane 1: F9 whole cell lysates
Lane 2: mouse liver lysates
Lane 3: NIH/3T3 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 44 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

44001

forkhead box H1

forkhead box protein H1

Forkhead box protein H1

Fast1; Fast2; Fast-1; Fast-2??[Similar Products]

FOXH1_MOUSE

FOXH1: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Cellular Component: transcription factor complex; nuclear chromatin; nucleus

Molecular Function: protein domain specific binding; protein binding; androgen receptor binding; DNA binding; sequence-specific DNA binding; bHLH transcription factor binding; SMAD binding; transcription factor activity

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; embryonic heart tube anterior/posterior pattern formation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of estrogen receptor signaling pathway; anterior/posterior pattern formation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; axial mesoderm development; positive regulation of transcription from RNA polymerase II promoter; heart looping; determination of left/right symmetry; cell differentiation

FOXH1: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator

Cellular Component: nuclear chromatin; nucleus; transcription factor complex

Molecular Function: androgen receptor binding; bHLH transcription factor binding; DNA binding; protein binding; protein domain specific binding; sequence-specific DNA binding; SMAD binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; axial mesoderm development; cell differentiation; determination of left/right symmetry; embryonic heart tube anterior/posterior pattern formation; heart looping; negative regulation of estrogen receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway

Labbe E.,et al.Mol. Cell 2:109-120(1998). Liu B.,et al.Mol. Cell. Biol. 19:424-430(1999). Weisberg E.,et al.Mech. Dev. 79:17-27(1998). Chen Y.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.

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