Full Product Name
LCA5 siRNA (Human)
Product Synonym Names
C6orf152; Lebercilin; Leber congenital amaurosis 5 protein
Product Gene Name
LCA5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q86VQ0
Specificity
LCA5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LCA5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LCA5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LCA5 sirna
siRNA to inhibit LCA5 expression using RNA interference
Applications Tested/Suitable for LCA5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LCA5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001116241.1
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NCBI GenBank Nucleotide #
NM_001122769.2
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UniProt Primary Accession #
Q86VQ0
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UniProt Secondary Accession #
Q9BWX7; E1P542[Other Products]
UniProt Related Accession #
Q86VQ0[Other Products]
Molecular Weight
80,554 Da
NCBI Official Full Name
lebercilin
NCBI Official Synonym Full Names
Leber congenital amaurosis 5
NCBI Official Symbol
LCA5??[Similar Products]
NCBI Official Synonym Symbols
C6orf152
??[Similar Products]
NCBI Protein Information
lebercilin
UniProt Protein Name
Lebercilin
UniProt Synonym Protein Names
Leber congenital amaurosis 5 protein
Protein Family
Lebercilin
UniProt Gene Name
LCA5??[Similar Products]
UniProt Synonym Gene Names
C6orf152??[Similar Products]
UniProt Entry Name
LCA5_HUMAN
NCBI Summary for LCA5
This gene encodes a protein that is thought to be involved in centrosomal or ciliary functions. Mutations in this gene cause Leber congenital amaurosis type V. Alternatively spliced transcript variants are described. [provided by RefSeq, Oct 2009]
UniProt Comments for LCA5
LCA5: Might be involved in minus end-directed microtubule transport. Defects in LCA5 are the cause of Leber congenital amaurosis type 5 (LCA5). LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. Belongs to the LCA5 family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 6q14.1
Cellular Component: cytoplasm; microtubule organizing center; cilium
Molecular Function: protein binding; protein complex binding
Biological Process: protein transport; photoreceptor cell maintenance; intraflagellar transport
Disease: Leber Congenital Amaurosis 5
Research Articles on LCA5
1. Identification of novel LCA5 mutations in patients with Leber congenital amaurosis and retinitis pigmentosa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
