Product Name
NPBWR1, siRNA
Full Product Name
NPBWR1 siRNA (Mouse)
Product Synonym Names
GPR7; Neuropeptides B/W receptor type 1; G-protein coupled receptor 7
Product Gene Name
NPBWR1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49681
Specificity
NPBWR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NPBWR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NPBWR1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NPBWR1 sirna
siRNA to inhibit NPBWR1 expression using RNA interference
Applications Tested/Suitable for NPBWR1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NPBWR1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034472.1
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NCBI GenBank Nucleotide #
NM_010342.1
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UniProt Primary Accession #
P49681
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UniProt Related Accession #
P49681[Other Products]
Molecular Weight
36,076 Da
NCBI Official Full Name
neuropeptides B/W receptor type 1
NCBI Official Synonym Full Names
neuropeptides B/W receptor 1
NCBI Official Symbol
Npbwr1??[Similar Products]
NCBI Official Synonym Symbols
Gpr7; Nbpwr1
??[Similar Products]
NCBI Protein Information
neuropeptides B/W receptor type 1
UniProt Protein Name
Neuropeptides B/W receptor type 1
UniProt Synonym Protein Names
G-protein coupled receptor 7
Protein Family
Neuropeptides B/W receptor
UniProt Gene Name
Npbwr1??[Similar Products]
UniProt Synonym Gene Names
Gpr7??[Similar Products]
UniProt Entry Name
NPBW1_MOUSE
UniProt Comments for NPBWR1
NPBWR1: Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: neuron projection; membrane; integral to plasma membrane; plasma membrane; integral to membrane
Molecular Function: G-protein coupled receptor activity; neuropeptide binding; neuropeptide receptor activity; signal transducer activity
Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; G-protein signaling, coupled to cyclic nucleotide second messenger; neuropeptide signaling pathway; behavior; regulation of metabolic process; signal transduction
Research Articles on NPBWR1
1. Important roles of NPBWR1 in regulating amygdala function during physical and social stress.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
