Full Product Name
GDF1 siRNA (Mouse)
Product Synonym Names
GDF-1; Embryonic growth/differentiation factor 1; GDF-1
Product Gene Name
GDF1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20863
Specificity
GDF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GDF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GDF1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GDF1 sirna
siRNA to inhibit GDF1 expression using RNA interference
Applications Tested/Suitable for GDF1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GDF1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001156754.1
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NCBI GenBank Nucleotide #
NM_001163282.2
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UniProt Primary Accession #
P20863
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UniProt Secondary Accession #
Q6AXH1[Other Products]
UniProt Related Accession #
P20863[Other Products]
Molecular Weight
38,660 Da
NCBI Official Full Name
embryonic growth/differentiation factor 1
NCBI Official Synonym Full Names
growth differentiation factor 1
NCBI Official Symbol
Gdf1??[Similar Products]
NCBI Official Synonym Symbols
Gdf-1; AI385651
??[Similar Products]
NCBI Protein Information
embryonic growth/differentiation factor 1
UniProt Protein Name
Embryonic growth/differentiation factor 1
Protein Family
Embryonic growth/differentiation factor
UniProt Gene Name
Gdf1??[Similar Products]
UniProt Synonym Gene Names
Gdf-1; GDF-1??[Similar Products]
UniProt Entry Name
GDF1_MOUSE
NCBI Summary for GDF1
This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and ***** tissues. This protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a monocistronic mRNA early in development, and from a bicistronic mRNA in later stages that also encodes the LAG1 homolog, ceramide synthase 1 gene. [provided by RefSeq, Jul 2009]
UniProt Comments for GDF1
GDF1: May mediate cell differentiation events during embryonic development. Defects in GDF1 are a cause of conotruncal heart malformations (CTHM). A group of congenital heart defects involving the outflow tracts. Examples include truncus arteriosus communis, double-outlet right ventricle and transposition of great arteries. Truncus arteriosus communis is characterized by a single outflow tract instead of a separate aorta and pulmonary artery. In transposition of the great arteries, the aorta arises from the right ventricle and the pulmonary artery from the left ventricle. In double outlet of the right ventricle, both the pulmonary artery and aorta arise from the right ventricle. Defects in GDF1 are the cause of transposition of the great arteries dextro-looped type 3 (DTGA3). A congenital heart defect consisting of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. The presence or absence of associated cardiac anomalies defines the clinical presentation and surgical management of patients with transposition of the great arteries. Defects in GDF1 are a cause of tetralogy of Fallot (TOF). A congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. In this condition, blood from both ventricles (oxygen-rich and oxygen-poor) is pumped into the body often causing cyanosis. Belongs to the TGF-beta family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Molecular Function: cytokine activity; transforming growth factor beta receptor binding
Biological Process: regulation of apoptosis; BMP signaling pathway; in utero embryonic development; regulation of MAPKKK cascade; mesoderm development; signal transduction; cell development; endoderm development
Research Articles on GDF1
1. This implies that Gdf1 potentiates Nodal activity by stabilizing a low molecular weight fraction that is susceptible to neutralization by soluble Acvr2.
Precautions
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