Product Name
Ctsa, Recombinant Protein
Full Product Name
Ctsa, 24-474aa, Mouse, His tag, Baculovirus
Product Synonym Names
lysosomal protective protein isoform b; Ctsa; Cathepsin A; AU019505; PPCA; Ppgb
Product Gene Name
Ctsa recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
APDQDEIDCL PGLAKQPSFR QYSGYLRASD SKHFHYWFVE SQNDPKNSPV VLWLNGGPGC SSLDGLLTEH GPFLIQPDGV TLEYNPYAWN LIANVLYIES PAGVGFSYSD DKMYVTNDTE VAENNYEALK DFFRLFPEYK DNKLFLTGES YAGIYIPTLA VLVMQDPSMN LQGLAVGNGL ASYEQNDNSL VYFAYYHGLL GNRLWTSLQT HCCAQNKCNF YDNKDPECVN NLLEVSRIVG KSGLNIYNLY APCAGGVPGR HRYEDTLVVQ DFGNIFTRLP LKRRFPEALM RSGDKVRLDP PCTNTTAPSN YLNNPYVRKA LHIPESLPRW DMCNFLVNLQ YRRLYQSMNS QYLKLLSSQK YQILLYNGDV DMACNFMGDE WFVDSLNQKM EVQRRPWLVD YGESGEQVAG FVKECSHITF LTIKGAGHMV PTDKPRAAFT MFSRFLNKEP YVEHHHHHH
3D Structure
ModBase 3D Structure for P16675
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of Ctsa recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Ctsa recombinant protein
CTSA, also known as lysosomal protective protein isoform b, is a component of the lysosomal multienzyme complex along with beta-galactosidase and sialidase Neu1. It is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. Recombinant mouse CTSA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Ctsa recombinant protein
Signal Transduction; Metabolism
Applications Tested/Suitable for Ctsa recombinant protein
SDS-PAGE
SDS-Page of Ctsa recombinant protein
NCBI/Uniprot data below describe general gene information for Ctsa. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001033581
[Other Products]
NCBI GenBank Nucleotide #
NM_001038492.2
[Other Products]
UniProt Primary Accession #
P16675
[Other Products]
UniProt Secondary Accession #
Q8VEF6[Other Products]
UniProt Related Accession #
P16675[Other Products]
Molecular Weight
52.4kDa (459aa) 50-70kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
lysosomal protective protein isoform b preproprotein
NCBI Official Synonym Full Names
cathepsin A
NCBI Official Symbol
Ctsa??[Similar Products]
NCBI Official Synonym Symbols
PPCA; Ppgb; AU019505
??[Similar Products]
NCBI Protein Information
lysosomal protective protein
UniProt Protein Name
Lysosomal protective protein
UniProt Synonym Protein Names
Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; Protective protein cathepsin A; PPCA
Protein Family
Lysosomal protective protein
UniProt Gene Name
Ctsa??[Similar Products]
UniProt Synonym Gene Names
Ppgb; PPCA??[Similar Products]
NCBI Summary for Ctsa
This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
UniProt Comments for Ctsa
Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Product References and Citations for Ctsa recombinant protein
Seyrantepe V., et al. (2008) Circulation. 117:1973-1981.; Hiraiwa M., et al. (1999) Cell Mol Life Sci. 56:894-907.
Research Articles on Ctsa
1. in normal tissues the tandem of serine carboxypeptidases, Scpep1 and CathA likely constitutes an important part of the physiological mechanism responsible for the balanced elimination of heightened levels of ET-1 that otherwise would accumulate in tissues and consequently contribute to development of the hyper-proliferative corneal dystrophy and abnormal skin thickening
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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