Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT; TNNT2; Troponin T2, cardiac type

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

No cross reactivity with other proteins.

Immunogen Affinity Purified

Lyophilized

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-TNNT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for Cardiac Troponin T detection. Tested with WB, Direct ELISA in Human; Mouse; Rat.
Background: Cardiac muscle troponin T (cTnT), is a protein which in humans is encoded by the TNNT2 gene. It is mapped to 1q32.1. The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.

Western Blot (WB), Direct ELISA (EIA)

WB: 0.1-0.5 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.

Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.

35,794 Da

troponin T2, cardiac type

troponin T, cardiac muscle

Troponin T, cardiac muscle

TnTc; cTnT??[Similar Products]

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

1. Aviles, R. J., Askari, A. T., Lindahl, B., Wallentin, L., Jia, G., Ohman, E. M., Mahaffey, K. W., Newby, L. K., Califf, R. M., Simoons, M. L., Topol, E. J., Lauer, M. S. Troponin T levels in patients with acute coronary syndromes, with or without renal dysfunction. New Eng. J. Med. 346: 2047-2052, 2002. 2. Charlet-B, N., Logan, P., Singh, G., Cooper, T. A. Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Molec. Cell 9: 649-658, 2002. 3. Du, C.-K., Morimoto, S., Nishii, K., Minakami, R., Ohta, M., Tadano, N., Lu, Q.-W., Wang, Y.-Y., Zhan, D.-Y., Mochizuki, M., Kita, S., Miwa, Y., Takahashi-Yanaga, F., Iwamoto, T., Ohtsuki, I., Sasaguri, T. Knock-in mouse model of dilated cardiomyopathy caused by troponin mutation.Circ. Res. 101: 185-194, 2007.

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