Product Name
NLRP12, Polyclonal Antibody
Popular Item
Full Product Name
NLRP12 Polyclonal Antibody
Product Synonym Names
CLR19.3; FCAS2; NALP12; PAN6; PYPAF7; RNO; RNO2
Product Gene Name
anti-NLRP12 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
LWLKICRLTA AACDELASTL SVNQSLRELD LSLNELGDLG VLLLCEGLRH PTCKLQTLRL GICRLGSAAC EGLSVVLQAN HNLRELDLSF NDLGDWGLWL LAEGLQHPAC RLQKLWLDSC GLTAKACENL YFTLGINQTL TDLYLTNNAL GDTGVRLLCK RLSHPGCKLR VLWLFGMDLN KMTHSRLAAL RVTKPYLDIG C
3D Structure
ModBase 3D Structure for P59046
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human NLRP12
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm
Positive Samples
Rat spleen
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-NLRP12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NLRP12 antibody
This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-NLRP12 antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-NLRP12 antibody
Western Blot (WB)
Application Notes for anti-NLRP12 antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-NLRP12 antibody
Western blot analysis of extracts of rat spleen, using NLRP12 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
NCBI/Uniprot data below describe general gene information for NLRP12. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001264055.1
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NCBI GenBank Nucleotide #
NM_001277126.1
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UniProt Primary Accession #
P59046
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UniProt Secondary Accession #
Q8NEU4; Q9BY26; A8MTQ2; B3KTE7[Other Products]
UniProt Related Accession #
P59046[Other Products]
Molecular Weight
Calculated: 31kDa; 101kDa; 107kDa; 113kDa; 114kDa; 120kDa
Observed: 110kDa
NCBI Official Full Name
NACHT, LRR and PYD domains-containing protein 12 isoform 3
NCBI Official Synonym Full Names
NLR family pyrin domain containing 12
NCBI Official Symbol
NLRP12??[Similar Products]
NCBI Official Synonym Symbols
RNO; PAN6; RNO2; FCAS2; NALP12; PYPAF7; CLR19.3
??[Similar Products]
NCBI Protein Information
NACHT, LRR and PYD domains-containing protein 12
UniProt Protein Name
NACHT, LRR and PYD domains-containing protein 12
UniProt Synonym Protein Names
Monarch-1; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide
Protein Family
NACHT, LRR and PYD domains-containing protein
UniProt Gene Name
NLRP12??[Similar Products]
UniProt Synonym Gene Names
NALP12; PYPAF7; RNO??[Similar Products]
NCBI Summary for NLRP12
This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Comments for NLRP12
May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK.
Research Articles on NLRP12
1. all of the reported mutations were found to have occurred in a highly conserved region in the NACHT domain coding sequence in NLRP12 exon 3, suggesting that a screening strategy for Familial cold autoinflammatory syndrome should focus on this area of the gene
Precautions
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Disclaimer
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