RP11; PRP31; SNRNP61; NY-BR-99

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-PRPF31 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western blot analysis of extracts of various cell lines, using PRPF31 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

499

pre-mRNA processing factor 31

U4/U6 small nuclear ribonucleoprotein Prp31; hPrp31; protein 61K; U4/U6 snRNP 61 kDa protein; pre-mRNA-processing factor 31; PRP31 pre-mRNA processing factor 31 homolog; serologically defined breast cancer antigen NY-BR-99

U4/U6 small nuclear ribonucleoprotein Prp31

PRP31; Protein 61K; hPrp31??[Similar Products]

PRP31_HUMAN

This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.[provided by RefSeq, Jan 2009]

PRPF31: Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation. Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant. Belongs to the PRP31 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: Cajal body; U4/U6 x U5 tri-snRNP complex; nuclear speck; nucleus

Molecular Function: ribonucleoprotein binding; protein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome

Disease: Retinitis Pigmentosa 11

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