Human AP-2 complex subunit alpha-2 (AP2A2) ELISA kit; ADTAB; CLAPA2; HIP9; HYPJ; adaptin; alpha B; alpha-adaptin C; Huntingtin interacting protein J; clathrin-associated/assembly/adaptor protein; large; alpha 2; adaptor-related protein complex 2; alpha 2 subunit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AP2A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9336545 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adaptor-related protein complex 2, alpha 2 subunit (AP2A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP2A2. The ELISA analytical biochemical technique of the MBS9336545 kit is based on AP2A2 antibody-AP2A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP2A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP2A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

103,960 Da

adaptor-related protein complex 2, alpha 2 subunit

AP-2 complex subunit alpha-2; alpha2-adaptin; adaptin, alpha B; huntingtin yeast partner J; 100 kDa coated vesicle protein C; huntingtin-interacting protein 9; adapter-related protein complex 2 subunit alpha-2; alpha-adaptin C; Huntingtin interacting protein J; clathrin assembly protein complex 2 alpha-C large chain; plasma membrane adaptor HA2/AP2 adaptin alpha C subunit; clathrin-associated/assembly/adaptor protein, large, alpha 2

AP-2 complex subunit alpha-2

ADTAB; CLAPA2; HIP9; HYPJ; KIAA0899; HIP-9??[Similar Products]

AP2A2_HUMAN

AP2A2: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes large subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasmic membrane-bound vesicle; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein binding; lipid binding; protein kinase binding; protein transporter activity

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; axon guidance; intracellular protein transport; synaptic transmission; nerve growth factor receptor signaling pathway; viral reproduction; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.