PYHIN4; Interferon-inducible protein AIM2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Absent In Melanoma 2 (AIM2).
No significant cross-reactivity or interference between Absent In Melanoma 2 (AIM2) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AIM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2020469 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Absent In Melanoma 2 (AIM2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AIM2. The ELISA analytical biochemical technique of the MBS2020469 kit is based on AIM2 antibody-AIM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AIM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AIM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Absent In Melanoma 2 (AIM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Absent In Melanoma 2 (AIM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Absent In Melanoma 2 (AIM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Absent In Melanoma 2 (AIM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tumor immunity

38,954 Da

absent in melanoma 2

interferon-inducible protein AIM2

Interferon-inducible protein AIM2

AIM2_HUMAN

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]

AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; double-stranded DNA binding

Biological Process: apoptosis; positive regulation of interleukin-1 beta secretion; activation of NF-kappaB transcription factor; positive regulation of protein oligomerization; positive regulation of interleukin-1 beta production; tumor necrosis factor-mediated signaling pathway; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta secretion; immune response; inflammatory response; positive regulation of defense response to virus by host; activation of innate immune response

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.