Product Name
WDR72, siRNA
Full Product Name
WDR72 siRNA (Human)
Product Synonym Names
WD repeat-containing protein 72
Product Gene Name
WDR72 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q3MJ13
Specificity
WDR72 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WDR72 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of WDR72 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
WDR72 sirna
siRNA to inhibit WDR72 expression using RNA interference
Applications Tested/Suitable for WDR72 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for WDR72. It may not necessarily be applicable to this product.
NCBI Accession #
NP_877435.3
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NCBI GenBank Nucleotide #
NM_182758.3
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UniProt Primary Accession #
Q3MJ13
[Other Products]
UniProt Secondary Accession #
Q7Z3I3; Q8N8X2[Other Products]
UniProt Related Accession #
Q3MJ13[Other Products]
Molecular Weight
123,425 Da
NCBI Official Full Name
WD repeat-containing protein 72 isoform a
NCBI Official Synonym Full Names
WD repeat domain 72
NCBI Official Symbol
WDR72??[Similar Products]
NCBI Official Synonym Symbols
AI2A3
??[Similar Products]
NCBI Protein Information
WD repeat-containing protein 72
UniProt Protein Name
WD repeat-containing protein 72
Protein Family
WD repeat-containing protein
UniProt Gene Name
WDR72??[Similar Products]
UniProt Entry Name
WDR72_HUMAN
NCBI Summary for WDR72
This gene encodes a protein with eight WD-40 repeats. Mutations in this gene have been associated with amelogenesis imperfecta hypomaturation type 2A3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Comments for WDR72
WDR72: Defects in WDR72 are the cause of amelogenesis imperfecta hypomaturation type 2A3 (AI2A3). A defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
Chromosomal Location of Human Ortholog: 15q21.3
Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia3
Research Articles on WDR72
1. WDR72 has a major role in enamel mineralization, most notably during the maturation stage, suggesting a function involving endocytic vesicle trafficking, and the removal of amelogenin proteins.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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