CECR1/Adenosine deaminase CECR1/Cat eye syndrome critical region protein 1/ADA2/ADGF/IDGFL

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of CECR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS761620 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine deaminase CECR1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CECR1. The ELISA analytical biochemical technique of the MBS761620 kit is based on CECR1 antibody-CECR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CECR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CECR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CECR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CECR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CECR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CECR1 can be calculated.

30,668 Da

cat eye syndrome chromosome region, candidate 1

adenosine deaminase CECR1

Adenosine deaminase CECR1

ADA2; ADGF; IDGFL??[Similar Products]

CECR1_HUMAN

This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

CECR1: Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity. Belongs to the adenosine and AMP deaminases family. ADGF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Hydrolase; EC 3.5.4.4

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: extracellular region; extracellular space

Molecular Function: adenosine deaminase activity; adenosine receptor binding; growth factor activity; heparin binding; protein homodimerization activity; proteoglycan binding; zinc ion binding

Biological Process: adenosine catabolic process; cellular protein metabolic process; hypoxanthine salvage; inosine biosynthetic process; multicellular organismal development

Disease: Polyarteritis Nodosa, Childhood-onset; Sneddon Syndrome

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