Mouse Acetylcholinesterase (AChE) ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; Acetylcholinesterase (AChE)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse AChE. No significant cross-reactivity or interference between mouse AChE and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AChE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS944406 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholinesterase (AChE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AChE. The ELISA analytical biochemical technique of the MBS944406 kit is based on AChE antibody-AChE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AChE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AChE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.

68,169 Da

acetylcholinesterase

acetylcholinesterase

Acetylcholinesterase

AChE??[Similar Products]

ACES_MOUSE

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Lipid Metabolism - glycerophospholipid; Hydrolase; Membrane protein, GPI anchor

Cellular Component: Golgi apparatus; extracellular space; neuron projection; cell surface; rough endoplasmic reticulum; endoplasmic reticulum lumen; dendrite; extracellular region; nuclear envelope; presynaptic membrane; postsynaptic membrane; membrane; cell soma; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; basal lamina; synapse; cell junction; neuromuscular junction

Molecular Function: collagen binding; choline binding; protein binding; serine hydrolase activity; protein homodimerization activity; cholinesterase activity; protein self-association; hydrolase activity; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: neurotransmitter catabolic process; acetylcholine metabolic process; acetylcholine catabolic process; positive regulation of dendrite morphogenesis; synaptogenesis; choline metabolic process; retina development in camera-type eye; receptor internalization; neurotransmitter receptor biosynthetic process; regulation of receptor recycling; positive regulation of axonogenesis; cell adhesion; protein tetramerization

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